The isolation and subfractionation of plasma membrane from the cellular slime mould Dictyostelium discoideum
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The isolation and subfractionation of plasma membrane from the cellular slime mould Dictyostelium discoideum.
A procedure for the isolation and separation of three different subfractions of plasma membrane from the cellular slime mould Dictyostelium discoideum is described. The cells were disrupted by freeze-thawing in liquid N(2) and plasma membranes were purified by equilibrium centrifugation in a sucrose gradient. The cell surface was labelled with radioactive iodide by using the lactoperoxidase iod...
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The effects of low temperature on morphogenesis and cellular differentiation of Dictyostelium discoideum were examined. During incubation at 5 degrees C, the vegetative and preaggregation cells never developed, but cell masses at the aggregation or slug stage developed to form hemispherical, or dumbbell-shaped multicellular structures. By staining with FITC-antispore IgG, the structures formed ...
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R E ~ N , E. REYES and J. RUfZ-HERRERA Genus Candida. By A. LETH BAK and A. STENDERUP BRAITH and J. E. SMITH . By B. L. BRADY . LIPPINCOTT and B. B. LIPPINCOTT . Influence of Oxygen on Maltose Metabolism by Mucor rouxii. By A. FLORES-CARDeoxyribonucleic Acid Homology in Yeasts. Genetic Relatedness within the Sporulation of Aspergillus niger in Submerged Liquid Culture. By J. C. GALThe Acylamide...
متن کاملMotive force of the migrating pseudoplasmodium of the cellular slime mould Dictyostelium discoideum.
Motive forces of migrating pseudoplasmodia (slugs) of Dictyostelium discoideum were determined by application of a double-chamber method. The motive force of a whole slug was proportional to its volume, the value per unit volume being 5.85 x 10(-6) dyne/cm3 (58.5 N cm-3). The motive force was independent of temperature (13.5--26 degrees C) and decreased during prolonged migration. Motive force ...
متن کاملAnalysis of optical density wave propagation and cell movement in the cellular slime mould Dictyostelium discoideum
We have studied optical density wave propagation during aggregation of the cellular slime mould Dictyostelium discoideum in a quantitative manner by digital image analysis. The waves arc mostly single ended spiral waves starting from an aggregation center. We can measure a variety of parameters such as oscillation frequency, wave propagation velocity and wave shape. This allows the construction...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1974
ISSN: 0306-3283
DOI: 10.1042/bj1400313